This protocol is used for transcardial perfusion with HEPES-Sucrose Cutting Solution (Referred as HEPES hereafter), followed by fast brain dissection and flash freezing.

Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

AF0138_Mouse_Brain_Perfusion_and_Flash_Freezing_Protocol.docx